krasg12v  (Addgene inc)

Journal: BMC cancer

Article Title: First molecules to reactivate RAS G12V GTPase activity.

doi: 10.1186/s12885-025-13580-8

Figure Lengend Snippet: Fig. 1 The best- scored flexible docking poses of PER154A (A), PER160A (B), PER170A (C), and PER176A (D) docked to KRASG12V (PDB ID: 8AFD). Experi mental V12 and Q61 conformations have been shown in light gray, and results from flexible docking are shown in green. Hydrogen bonds are repre sented by dashed cyan lines

Article Snippet: Briefly, 500 ng of KRAS-WT or KRASG12V protein (both from SignalChem), 500 ng of GAP RT 01001-PER, molecules at selected concentrations – 10 μM, 1 μM DTT, 5 μM GDP and GTPase/GAP Buffer were applied to white non-transparent 96-well plates to a final volume of 25 μl per well.

Techniques:

Journal: BMC cancer

Article Title: First molecules to reactivate RAS G12V GTPase activity.

doi: 10.1186/s12885-025-13580-8

Figure Lengend Snippet: Fig. 3 Analysis of the GTPase activity of the KRASG12V protein in the presence of selected molecules. (A) Verification of the action of GAP family proteins – a commercially available and self-obtained GAP RT 01001-PER on KRASWT and KRASG12V proteins. No functionality was observed for the commercial pro tein, while GAP RT 01001-PER showed GTPase activity for KRASWT and had no effect on KRASG12V. The GTPase activity of the KRASG12V protein was partially restored by selected molecules. These effects were observed both in the absence (B) and presence (C) of the GAP RT 01001-PER protein

Article Snippet: Briefly, 500 ng of KRAS-WT or KRASG12V protein (both from SignalChem), 500 ng of GAP RT 01001-PER, molecules at selected concentrations – 10 μM, 1 μM DTT, 5 μM GDP and GTPase/GAP Buffer were applied to white non-transparent 96-well plates to a final volume of 25 μl per well.

Techniques: Activity Assay